This study was conducted to compare the effect of supplementation of prebiotic and probiotic on growth performance and nutrient digestibility in finishing pigs. One hundred and forty female pigs (75 ± 0.5 kg) were divided into 6 treatments with 3 replicates of twenty pigs each treatment. The experimental were analyzed by completely randomized design (CRD). The diets were composed by Treatment I) basal diet, Treatment II) basal diet mixture with 1.5 percentage of SuperYea, Treatment III) basal diet mixture with 0.2% of bacillus subtilis (1 x 1012 CFU), Treatment IV) basal diet mixture with 0.2% of Saccharomyces cerivisae (5 x 10⁹ CFU), Treatment V) basal diet mixture with 0.2% of bacillus subtilis (1 x 10¹² CFU) plus lactobacillus lactic (1 x 10¹¹ CFU) and Treatment VI) basal diet mixture with 0.2% of bacillus subtilis (1 x 10¹² CFU) plus Saccharomyces cerivisae (5 x 10⁹ CFU)and lactobacillus lactic (1 x 10¹¹ CFU).

The result of the experiment showed that pigs fed multi-strain microbial mixture with SuperYea of diet showed heavier final body weight than basal diet (96.4, 98.8, 98.1, 96.9, 99.2 and 99.6 kg/pig), body weight gain (20.9, 23.4, 22.5, 21.4, 23.8 and 24.2 kg/pig), average daily gain (698, 780, 766, 713, 793 and 806 g/d) and feed conversion ratio of supplementation multi-strain mixture with SuperYea of diet were lower than another treatments (p < 0.05). The dry matter digestibilities of multi-strain microbes higher (p < 0.05) than another treatments (86.4, 88.3, 87.4, 86.7, 86.53 and 89.13%), the crude protein digestibilities were better (p < 0.05) with supplementation of multi-strain microbial in diet than another treatments (73.4, 75.3, 74.1, 74.8, 75.6 and 76.5%). In conclusion, dietary inclusion of multi-strain microbes can be improved growth performance and nutrient digestibility in finishing pigs.

The use of antibiotic in disease prevention and growth performance can be bring about emergence of drug-resistant microorganisms and leave antibiotic residues in animal feed (Weston, 1996, Esiobu., et al. 2002, Azza., et al. 2009). So a lot of researcher try to studied on prebiotic or probiotic as new feed additive and can be improved growth performance and healthy for animal production.

Prebiotics are indigestible carbohydrates, which pass through small intestines and are broken down in the colon (Olsen and Maribo, 1999; Houdijk., et al. 1998). A macroorganism does not produce enzymes for breaking down of prebiotics. They are digested by bacteria of the digestive tract living in the colon. This action results formation of short-chain fatty acids, which in turn reduce pH in the colon, create unfavorable conditions for development of pathogenic bacteria (Poeikhampa., et al. 2011; Hinton., et al.1990).

Probiotics are live bacteria or yeast preparations containing microorganisms of one or several kinds, which have an antagonistic effect on pathogenic bacteria in intestines as well as a positive effect on microflora in intestines and the macro organism itself (Xuan., et al. 2001; Chang., et al. 2000; Vanbella., et al.1990).

The purpose of the present study is to investigate the effects of prebiotic and probiotic on growth performance and nutrient digestibility of finishing pigs.

This study was conducted at Nongbua Farm & Country Home Village Co., Ltd at Ratchaburi Province, Thailand, and experimental animals were kept, maintained and treated in adherence to accept standards for the humane treatment of animals.

SuperYea
The SuperYea is a one part of by-product from ethanol factory by molasses and yeast as substrate and the SuperYea has composes high concentration of minerals, low fiber and 15% of β-glucan and prebiotic source for animal (Tumwasorn, 2012).

Animal and Managements
Two hundred and forty male commercial crossbred piglets (Duroc x Large White x Landrace; 75.00 ± 0.50 kg body weight) were used in this trail. The pigs were divided into 6 treatments and each treatment consisted of 3 replications (twenty pigs per replication). The pigs were raised in naturally ventilated houses consisting of 18 pens (4 x 6m²), and each pen was assigned a crib and two of water nipples. During the feed trail, the piglets were bathed and the house was cleaned two days interval, while the face of piglets was removed every day.

Experimental design and diets
The Completely Randomized Design (CRD) was designed in this experiment. Three Experimental diets were provided to pigs for 3 weeks as follow; Treatment I) basal diet(Control), Treatment II) basal diet+1.5% of SuperYea, Treatment III) basal diet + 0.2% of Bacillus Subtilis (1 x 10¹² cfu), Treatment IV) basal diet + 0.2% of Saccharomyces cerivisae (5x10⁹ cfu), Treatment V) basal diet +0.2% of Bacillus Subtilis (1 x 10¹² cfu) + Lactobacillus Lactic (1 x 10¹¹ cfu) and Treatment VI) basal diet + 0.2% of Bacillus Subtilis (1 x 10¹² cfu) + Lactobacillus Lactic (1 x 10¹¹ cfu) + Saccharomyces cerivisae (5 x 10⁹ cfu). The basal diets were formulated to provide the same amount of nutrients and net the requirement by National Research Council (NRC, 1998) as shown in Table 1. Feed and water were provided ad libitum. Body weight and feed intake were recorded one week interval.

Premix content; Vitamin A 4MIU, D 0.65 MIU, E 24,000 IU, K₃1.4g, B₁ 0.6g, B₂ 0.3g, B₆ 0.75g, B₁₂ 14 mg, Nicotinic 20g, Pantothenic acid 10g, Folic acid 0.44g, Biotin 0.04g, Choline 60g, Fe 45g, Cu 40g, Mn 15g, Zn 40g, Co 0.2g, I 0.4g, Se 0.06g, Carrier Added to 1 kg.

Parameters
Growth Performance: Theinitial body weight of each pigs was recorded and at the end of feeding trail (9 weeks) the body weight, body weight gain and feed intake were recorded one week interval in order to calculation of average daily gain, average daily feed intake and feed to gain ratio.

Nutrient Digestibility: One week before the end of experiment, Chromium oxide (Cr₂0₃)was added at 0.2 percentages of the diet as an indigestible marker to calculate digestibility coefficient. Fecal samples were randomly drawn from each treatment around 30%. After collection, samples will be frozen and stored in refrigerator at -20°c until analysis take place. Before determination of dry matter (DM), crude protein (CP), crude fiber (CF) and ether extracts (EE) analyzed according to AOAC (AOAC, 1994) and chromium will be analyzed by UV absorption spectrophotometry (Shimadzu, UV1201, Japan).

Statistical analysis
  All Data were statistically analyzed using analysis of covariance (ANOCOVA) of SAS (SAS, 1996). The differences between the means of groups were separated by Ducan’s New Multiple Range Test according to the following model:

Y_ij = µ + Ai + β (Wt_j-Wt_j) + Є_ij
Where;
Y_ij = observation of dependent variables from treatment i and replication j.
µ = the overall mean.
A_i = effect of treatment i^th (i = 1, 2, 3).
Β = coefficient regression of initial of body weight for experimental.
Wt_j = initial body weight of animals experimental j.
Wt_j = average of initial body weight of animals experimental j.
Є_ij = experimental error.

Statements of statistical significance were based on p < 0.05 and all data statistical analyses were done in accordance with the method of Steel and Torrie (1980).

Growth Performance
The growth performances of animals are shown in Table 2. The initial body weights of pigs were not significantly difference. At the end feeding trail, supplementation of prebiotic and probiotic are increase final body weight (FBW), body weight gain (BWG), average daily gain (ADG) and feed conversion ratio (FCR) than control treatments (p < 0.05).

Note:
T1: Control diet.
T2: add 1.50 percentage of SuperYea in the diet.
T3: add 0.20 percentage of bacillus Subtilis (1 x 10¹² CFU) in the diet.
T4: add 0.20 percentage of saccharomyces cerivisae (5 x 10⁹ CFU) in the diet.
T5: add 0.20 percentage of bacillus subtilis (1 x 10¹² CFU) mixture with lactobacillus lactic (1 x 10¹¹ CFU) in diet.
T6: add 0.20 percentage of bacillus subtilis (1 x 10¹² CFU)mixture with Lactobacillus lactic (1 x 10¹¹ CFU)and plus saccharomyces cerivisae (5 x 10⁹ CFU) in diet.
IBW: Initial Body Weight
FBW: Final Body Weight
BWG: Body Weight Gain
ADG: Average Daily Gain.
ADFI: Average Daily Feed Intake.
FCR: Feed Conversion Ratio
^abcMeans in the same row with different superscripts differ (P < 0.05).

Improvement of growth rate by feed additives supplementation confirms positive effect of this prebiotic that has previously reported by Chen., et al. (2006), also observed an improvement when growing pig fed diets supplemented complex probiotic (Lactobacillus acidophilus, Saccharomyces cerevisiae and Bacillus subtilis). Alexopoulos., et al. (2004) observed significant improvement when finishing pigs fed diet included probiotic (Bacillus licheniformis and Bacillus subtilis). Huang., et al. (2004) using a complex Lactobacilli preparation also observed improvements in growth performance of nursery pigs. When probiotics are added to growing or finishing pig diets, Jonsson and Conway (1992), who reported dietary addition of bacillus species improved growth performance and health of pigs and Cho., et al. (1992) reported that supplementation of Lactobacillus casei in diets improved growth performance of piglets and appeared to be more effective than sub-therapeutic antibiotics. However, Kornegay., et al. (1990) reported that there was no effect on growth performance by the supplementation of Lactobacillus acidophilus in finishing pigs. Unlike the diverse results obtained from growing and finishing pig experiments, many studies of probiotics conducted in nursery pigs found positive effects when diets added probiotic preparations (Lessard and Brissom, 1987; Park., et al. 2001).

Nutrient digestibility
The nutrient digestibilities of animals are shown in Table 3. The crude fiber, ether extract and crude ash were significantly difference on supplementation of prebiotic and probiotic in diets than control diet (p < 0.05).

Note:
T1: basal diet.
T2: add 1.50 percentage of SuperYea in the diet.
T3: add 0.20 percentage of bacillus Subtilis (1 x 10¹² CFU) in the diet.
T4: add 0.20 percentage of saccharomyces cerivisae (5 x 10⁹ CFU) in the diet.
T5: add 0.20 percentage of bacillus subtilis (1 x 10¹² CFU) mixture with lactobacillus lactic
(1 x 10¹¹ CFU) in diet.
T6: add 0.20 percentage of bacillus subtilis (1 x 10¹² CFU)mixture with Lactobacillus lactic
(1 x 10¹¹ CFU)and plus saccharomyces cerivisae (5 x 10⁹ CFU) in diet.
^abcMeans in the same row with different superscripts differ (P < 0.05).

Nutrient digestibility of various feed mixed with prebiotic and probiotic in this study showed the positive prebiotic mixed with probiotic effect as reported by Shen., et al. (2014) was reported dry matter and crude protein (P < 0.05) was increased digestibility’s by dietary supplementation of yeast culture and antibiotic growth promoter than control diet. This study Bacillus-based feed additive on nutrient digestibility was increased diegestibilities for dry matter and crude protein when supplementation than control (p < 0.05). Zhao and Kim (2013) reported of study by direct-fed 0.1% L. reuteri and L. plantarum complex (1 × 109 cfu/kg in diets can be improved digestibilities of dry matter and crude protein than control (p < 0.05).

For nutrient digestibility and absorption capacity of the small intestine was affected by villus: crypt ratio (Montagne., et al. 2003). This study demonstrated that dietary supplementation of yeast culture improved the digestibility of dry matter and crude protein which may be due to increased villus height and villus: crypt ratio in the jejunum. However, these results are not consistent with other studies. Kornegay., et al. (1995) observed that the digestibility of protein and energy was not affected by yeast culture supplementation.

The result of this study suggested that supplementation of multri-strain probiotic mixture with SuperYea as based feed additives in finishing pigs significantly improved final weight gain, body weight gain, average daily gain, feed conversion ratio, dry matter digestibility, and crude protein digestibility.

Acknowledgement
The authors gratefully acknowledge funding from the Rich and Green Co., Ltd, Thailand. The supporting from Nongbua Farm & Country Home Village Co., Ltd, Ratchaburi Province, Thailand for suggestion, guidance, throughout this trail was graceful. We thank the Thailand International Development Cooperation Agency (TICA) under program “Ayeyawady-Chao Praya-Mekong Economic Cooperation Strategy (ACMECS)”, Ministry of Foreign Affair, Bangkok, Thailand.